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Ames Testing

AMES™ Mutagenicity Assays

AMES MPF™ Literature

AMES II™ Literature

PETRIFILM Literature

 

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Ames MPF™
Lab Worker Looking Through a Microscope   Microplate of Xenometrix Testing
  • Semi-Solid and Liquid format; 384-well microplates.
  • Kits can be ordered witht Aroclor 1254 Induced Lyophilized or Phenobarbital/Beta Naphtoflavone Induced Rat Liver S9 and Positive Controls.
  • Significantly decreases compound consumption; increases assay through-put.
  • Time and cost-effective pre-regulatory alternative to plate incorporation method.
  • Kit includes ready-to-use media and bacteria.
  • Enables rapid screening of a large number of compounds.
  • Petrifilm aqua products are now available.
  • New Products XenoScreen YES/YAS Test Kits are now available for the detection of estrogenic and androgenic endocrine disruptors.

Ames MPF™ Kits (Semi-Solid) Ships at Room Temperature
The Ames MPF™ Semi-Solid assays are modified versions of the Ames test using semi-solid(ships at room temperature) culture instead of agar plates. The assays are based on the fluctuation method cited in the OECD Guideline 471 but use 384-well microtiter plates and have a colorimetric read-out. The great advantage of this format is the lower amount of sample needed and much easier handling. The Ames MPF™ assays use TA98 and/or TA100, TA1535, TA1537. All these tester strains are recommended in the current, formal test guidelines.

Ames UmuC Easy AQ and UmuC Easy CS Test Kits
The UmuC Easy AQ and UmuC Easy CS – SOS response test kits for assessing genotoxic activity Xenometrix AG, Ready-to-use kits for the detection of potentially genotoxic compounds by induction of the SOS response in Salmonella typhimurium TA1535 [pSK1002].

Ames MPF™ 98-100 Aqua Testing
The novel Ames MPF™ 98/100 AQUA is designed to detect the mutagenic potential of non-concentrated water samples. The assay allows to use 18.5 times more sample volume than with the conventional Ames MPF™ 98/100 for testing of chemical compounds thanks to a 10-fold concentrated Exposure Medium. The assay uses S. typhimurium TA98 and TA100 strains and may be used for the testing of sterile aqueous solutions, such as fresh water, waste water, aqueous extracts and leachates, aqueous solutions of chemicals and chemical mixtures.

Ames MPF™ YG Test Kits
The novel Ames MPF™ 98/100 AQUA is designed to detect the mutagenic potential of non-concentrated water samples. The assay allows to use 18.5 times more sample volume than with the conventional Ames MPF™ 98/100 for testing of chemical compounds thanks to a 10-fold concentrated Exposure Medium. The assay uses S. typhimurium TA98 and TA100 strains and may be used for the testing of sterile aqueous solutions, such as fresh water, waste water, aqueous extracts and leachates, aqueous solutions of chemicals and chemical mixtures.

XenoScreen YES/YAS Assay Kits

New Products XenoScreen YES/YAS Assay kit for the detection of estrogenic and androgenic endocrine disruptors.

The advantages of the XenoScreen YES/YAS assay kit are:

  • YES (estrogen) and YAS (androgen) analysis in 1 kit.
  • Simultaneous analysis of activating (agonist) and inhibiting (antagonist) activities.
  • High sensitivity (YES: <1 x 10-10 M, YAS <1 x 10-9 M).
  • Assay time: 48 hrs.
  • Microplate format, colorimetric read-out.
  • Step-by-step Instruction Manual for first-time success.
  • Free customer support.
  • Excel calculation sheet for data analysis.

Ames Testing Plasticware
Plastic ware to be used with our Ames II™ and Ames MPF™ kits.

Ames MPF™/Ames II™ Media (Ready to use)
Growth, Exposure and Reversion Indicator Medium to be used with our Ames II™ and Ames MPF™ kits.

Ames MPF™/Ames II™ 10 Fold Exposure Medium
Ames MPF™/Ames II™ 10 Fold concentrated Exposure Medium to be used with our Ames II™ and Ames MPF™ kits.

Ames MPF™ E.coli Media (Ready to use)
Ames MPF™ E.coli Media - Ready to use.

Ames Liquid S. Typhimurium Strains
Ames MPF™ strains in liquid format. TA98, TA100, TA1535, TA1537 and TAMix. These strains are shipped with dry-ice.

Ames Semisolid S. Typhimurium Strains
Individual Ames MPF™ (Semi-Solid) strains TA98, TA100, TA1535 and TA 1537. These strains are shipped at room temperature.

Ames Liquid E.coli Strains
Individual Ames Liquid typhimurium E.coli strains uvrA and pkM101. Ames liquid E.coli Strains are shipped with dry ice.

Ames Semisolid E.coli Strains
Individual Ames Semisolid typhimurium E.coli strains uvrA and pkM101. Ames semisolid E.coli strains ship at room temperature.

Ames umuC Semisolid Strain
Individual Ames umuC Semisolid Strain. umuC Semisolid strain shipped at room temperature.

Ames Positive Controls
Positive controls for Ames testing.

Ames Rat Liver S9 With S9-100/1537 Booster Solution
Rat Liver S9 with S9-100/1537 Booster Solution for Ames testing. Recommended to reduce possible toxic effects of high S9 concentrations in TA100 and TA1537.

Ames S9 Cofactor Kit
Complete S9 activation system in a set of three ready-to-use reagents: a buffer-salt solution, a G-6-P solution and a NADP solution which are mixed at specified proportions with the S9 rat liver enzymes.

Analytical Services
Xenometrix has at its Allschwil facility a fully equipped laboratory for performing Ames MPF™ and Ames II Mutagenicity Assays for customers. Chemicals will be accepted to perform the required assays, interpret data and produce detailed reports. The staff is fully trained in all aspects of the liquid Ames microplate assays, making for a very efficient and cost-effective process. Depending upon answers to a client's questionnaire, detailed reports are likely to be completed within 7 days. However, data can be communicated immediately upon assay completion (3 days).

The Ames II™
Lab Worker Holding a Vial   Mutagenicity Assay, in liquid micro plate format, is a modification of the traditional Ames Plate Incorporation Assay and the Mini-Ames test. The Ames II™ test reduces hands-on time and also uses much less of the test compound than the regular Ames or Mini-Ames tests.
  • Microplate format
  • Ready-to-use reagents
  • Less hands-on time
  • Significantly lower amount of test compound
  • Automatable for high throughput screening
  • Six fold less plastic ware, reduced disposal costs

The Ames II™ Tests without Rat Liver S9

The Ames II™ Tests with Rat Liver S9

Ames II™ Strains
Individual Ames II™ strains TA98, TAMix and Ampicillin.

Ames MPF™/Ames II™ Media (Ready to use)
Growth, Exposure and Reversion Indicator Medium to be used with our Ames II and Ames MPF™ kits.

Petrifilm™ Products

Petrifilm™ Aqua Plates are sample-ready media that replace conventional agar, petri dishes, media pads and disposable filter funnels used in the microbial testing of water.

Each plate contains a water-soluble gelling agent, nutrients and indicators in a dry, shelf-stable format. We offer four 3M Petrifilm Aqua Plates to cover your unique testing needs:

  • Heterotrophic Count
  • Coliform Count
  • Enterobacteriaceae Count
  • Yeast & Mold Count

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